Deltamethrin activates transcriptional factors NRF2 but does not increase GCSH protein expression in primary astrocytes with laser scanning confocal microscope.
Li-H; Shi-N; Dai-Z; Zhong-Y; Ma-Q
Drug Metab Rev 2005 Jan; 37(Suppl 2):410
Recent studies show that activation of transcriptional factors Nrf2 is a key initiation step in the cellular response against chemical stress and Nrf2 involves in regulation of expression of gamma-glutamy1cysteine synthetase heavy (GCSh) and light subunit (GCSl). To our knowledge, however, there is no study reported whether disruptions in Nrf2 and GCS occur in neurotoxicity induced by Deltamethrin (DM). The cultured astrocytes were incubated with 10-5 mol/L DM and/or 40 umol/L tert-butylhydroquinone (tBHQ) for 1 or 6h, respectively. Both subcellular localization of Nrf2 and expression of GCSh was immunocytochemically detected by laser scanning confocal microscope. We found that DM can activate transcriptional activator Nrf2 in astrocytes. tBHQ not only induces Nrf2 expression but activates Nrf2. DM or/and tBHQ have no effect on the protein expression of GCSh in astrocytes.
Genes; Cellular-reactions; Proteins; Laboratory-animals; Animals; Animal-studies; Exposure-levels
Abstract; Conference/Symposia Proceedings
Drug Metabolism Reviews