Time course of gene expression of gamma-glutamylcysteine synthetase subunit and NRF2 in brain tissues of rats treated with deltamethrin.
Li-H; Shi-N; Dai-Z; Zhong-Y; Ma-Q
Drug Metab Rev 2005 Jan; 37(Suppl 2):409
Recent studies show that activation of transcriptional factors Nrf2 is a key initiation step in the cellular response against chemical stress and Nrf2 involves in regulation of expression of gamma-glutamylcysteine synthetase heavy (GCSh)and light subunit (GCSl). To our knowledge, however, there is no study reported whether disruptions in Nrf2 and y-GCS occur in neurotoxicity induced by Deltamethrin (DM). Wistar male rats were administrated with DM (single dose is respectively 0, 12.50 mg/Kg BWT) with i.p. for one time. At various time points post-exposure, the relative amount of mRNA expression of these genes was measured by the method of RTPCR and the protein level was detected by the method of immunohistochemistry and image analysis system. After single dose of DM exposure, there are up-regulation of mRNA expression of both GCSh and GCSl gene in rats hippocampus, down-regulation of mRNA expression of GCSh gene and up-regulation of mRNA expression of Nrf2 gene in rats cerebral cortex under the experimental condition. However, there is no obvious change on protein level of both GCSh and Nrf2 in hippocampus and cerebral cortex.
Genes; Cellular-reactions; Laboratory-animals; Animals; Animal-studies; Exposure-levels; Proteins
Abstract; Conference/Symposia Proceedings
Drug Metabolism Reviews