Application of Taqman chemistry for allelic discrimination.
Genetic Variance Detection: Technologies for Pharmacogenomics. Hecker-KH, ed., Eagleville, PA: DNA Press, 2005 Jan; :237-254
Taqman chemistry has been successfully adapted for use as a tool to discriminate allelic variation in human study populations. This technique has been validated against a number of well-accepted techniques including PCR-RFLP, sequencing, and allele-specific PCR. The Taqman assays are now being used as the basis for validation of newer genotyping strategies indicating its growing acceptance in the scientific literature. These assays are amenable to the high-throughput genotyping demands of the post-human genome sequencing era and are associated with less cost and technical error compared to more traditional approaches. The application of microfluidics and nanoliter volumes will greatly further the high-throughput/low cost attributes of this genotyping platform.
Genetics; Genotoxicity; Analytical-processes; Analytical-chemistry; DNA-damage; Bioassays; Biomarkers
Victor J. Johnson, Toxicology and Molecular Biology Branch, Health Effects Laboratory division, NIOSH/CDC, 1095 Willowdale Road, M/S 3014, Morgantown, WV 26505-2888
Book or book chapter
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Genetic Variance Detection: Technologies for Pharmacogenomics