Cell proliferation in over-loaded rat soleus muscles: myofiber splitting vs myogenesis.
Physiologist 1996 Oct; 39(5):A92
Injury to skeletal muscle has been associated with proliferation and migration of cells into regions with damaged myofibers. Many of these cells have been presumed to be inflammatory cells or phagocytes. Using two types of functional overload we have produced damage to myofibers which mimics overuse syndromes. By applying specific antibodies and using double-labeling techniques, we have identified macrophages, degenerating myofibers, fibroblasts, split myofibers and new myobalsts. Myoblasts can be visualized using desmin and dystrophin or desmin and vimentin. Fibroblasts stain only for vimentin. The location of central nuclei and intracellular deposition of vimentin, desmin and fibronectin appear to be related to myofiber splitting. Some myofibers which appear to have split contain different myosin isoforms whereas others are homogeneous.
Laboratory-animals; Animals; Animal-studies; Injuries; Skeletal-disorders; Skeletal-system; Muscles; Cell-migration; Phagocytes; Musculoskeletal-system-disorders
Departments of Physiology and Neurology, Robert C. Byrd Health Sciences Center, West Virginia University, PO Box 9229, Morgantown, WV 26506-9229
West Virginia University, Morgantown, West Virginia