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Characterization of nigerlysin©, hemolysin produced by Aspergillus niger, and effect of mouse neuronal cells in vitro.

Authors
Donohue-M; Wei-W; Wu-J; Zawia-NH; Hud-N; Jesus-VD; Schmechel-D; Hettick-JM; Beezhold-DH; Vesper-S
Source
Toxicology 2006 Feb; 219(1-3):150-155
NIOSHTIC No.
20029410
Abstract
Aspergillus niger produced a proteinaceous hemolysin, nigerlysin (c) when incubated on sheep's blood agar (SBA) at both 23 and 37 degrees C. Nigerlysin was purified from tryptic soy broth (TSB) culture filtrate and found to have a molecular weight of approximately 72kDa, with an isoelectric point of 3.45. Nigerlysin is heat stable up to 65 degrees C but unstable at 75 degrees C when incubated for 10min. Circular dichroic analysis revealed that nigerlysin has an alpha helical structure. Exposure of mouse primary cortical neuronal cells to 0.1mugml(-1) of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24h. The IC(50) is estimated to be 0.037mugml(-1), or between 0.034 and 0.041mugml(-1) at the 95% confidence level.
Keywords
Laboratory-animals; Animal-studies; Animals; In-vitro-studies; Exposure-levels; Exposure-assessment; Fungi; Fungicides; Pathogens; Infectious-diseases; Toxins; Toxic-effects; Mortality-data; Mortality-rates; Occupational-diseases; Occupational-exposure; Occupational-hazards
CODEN
TXCYAC
Publication Date
20060215
Document Type
Journal Article
Email Address
vesper.stephen@epa.gov
Fiscal Year
2006
Issue of Publication
1-3
ISSN
0300-483X
NIOSH Division
HELD
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Toxicology
State
WV; OH; GA; RI
Page last reviewed: May 5, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division