Characterization of nigerlysin©, hemolysin produced by Aspergillus niger, and effect of mouse neuronal cells in vitro.
Donohue-M; Wei-W; Wu-J; Zawia-NH; Hud-N; Jesus-VD; Schmechel-D; Hettick-JM; Beezhold-DH; Vesper-S
Toxicology 2006 Feb; 219(1-3):150-155
Aspergillus niger produced a proteinaceous hemolysin, nigerlysin (c) when incubated on sheep's blood agar (SBA) at both 23 and 37 degrees C. Nigerlysin was purified from tryptic soy broth (TSB) culture filtrate and found to have a molecular weight of approximately 72kDa, with an isoelectric point of 3.45. Nigerlysin is heat stable up to 65 degrees C but unstable at 75 degrees C when incubated for 10min. Circular dichroic analysis revealed that nigerlysin has an alpha helical structure. Exposure of mouse primary cortical neuronal cells to 0.1mugml(-1) of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24h. The IC(50) is estimated to be 0.037mugml(-1), or between 0.034 and 0.041mugml(-1) at the 95% confidence level.
Laboratory-animals; Animal-studies; Animals; In-vitro-studies; Exposure-levels; Exposure-assessment; Fungi; Fungicides; Pathogens; Infectious-diseases; Toxins; Toxic-effects; Mortality-data; Mortality-rates; Occupational-diseases; Occupational-exposure; Occupational-hazards
Research Tools and Approaches: Exposure Assessment Methods