Use of double labeling and photo CD for morphometric analysis of injured skeletal muscle.
Stauber WT; Miller GR; Gibala MJ; MacDougall JD
J Histochem Cytochem 1995 Nov; 43(11):1179-1184
We used computer-assisted analysis of myofiber cross-sectional areas to measure skeletal muscle responses to injury and disease. We developed a simple, inexpensive method for measuring myofiber size in human muscle samples using Kodak photo compact discs (CDs) as the image source. The photo CD serves as a permanent image storage medium and provides a high-resolution image that can be used to detect small myofibers. The use of double labeling for dystrophin and desmin allowed positive identification of both degenerating and regenerating fibers in a single biopsy specimen.
Injuries; Skeletal system disorders; Skeletal disorders; Musculoskeletal system disorders; Computer models; Computers; Diseases; Muscles; Sampling; Sampling methods
William T. Stauber, Ph. D., Department of Physiology, West Virginia University, P.O. Box 9229, Morgantown, WV 26507-9229
Journal of Histochemistry and Cytochemistry
West Virginia University, Morgantown, West Virginia