Cross-reactivity of monoclonal antibodies against aspergillus versicolor and other fungi and their implications for the development of antibody-based monitoring techniques for fungi.
Schmechel-D; Simpson-J; Lewis-DM
Bioaerosols, Fungi, Bacteria, Mycotoxins and Human Health: Patho-physiology, Clinical Effects, Exposure Assessment, Prevention and Control in Indoor Environments and Work, June 2005, Johanning E, ed., Albany, NY: Fungal Research Group Foundation, Inc., 2005 Jun; :366-374
In order to improve current culture-based monitoring techniques for Aspergillus versicolor and other fungi in general, our laboratory is developing species-specific monoclonal antibody (mAb)-based immunoassay techniques. In this study we attempted to enhance the production of mAbs specific for A. versicolor by immunizing mice with pre-formed immune complexes to block epitopes of spores known to be cross-reactive with other fungi. We found that immunization with immune complexes did not enhance the production of A. versicolor-specific mAbs. While the antibody reactivity to related Aspergillus and Penicillium species was reduced, the response to other fungi was enhanced. The patterns of cross-reactivity among the 50 tested fungal species not only demonstrate that phylogenetically distant fungi share common epitopes but also that different fungi express a given cross-reactive epitope in variable amounts on a per spore basis.
Fungi; Fungicides; Antibody-response; Laboratory-techniques; Laboratory-animals; Animals; Animal-studies; Monitoring-systems
National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, M/S H-4020, Morgantown, WV 26505, USA
Book or book chapter
Research Tools and Approaches: Exposure Assessment Methods
Bioaerosols, Fungi, Bacteria, Mycotoxins and Human Health: Patho-physiology, Clinical Effects, Exposure Assessment, Prevention and Control in Indoor Environments and Work