A test procedure for the determination of (2-methoxyethoxy) acetic acid in urine from jet fuel-exposed mice.
B'Hymer C; Keil DE; Cheever KL
Toxicol Mech Methods 2005 Sep; 15(5):367-373
A test procedure for the determination of (2-methoxyethoxy)acetic acid (MEAA) was adapted and applied to urine samples from jet fuel (JP-8)-exposed mice using capillary gas chromatography with a mass selective detector (MSD). MEAA is a metabolite and proposed biomarker for exposure to 2-(2-methoxyethoxy)ethanol, a glycol ether component in the formulation of JP-8. The collected urine samples were spiked with deuterated butoxyacetic acid internal standard, and extracted with ethyl acetate, and esterified with ethanol and sulfuric acid, and the esters of the glycol ethers were extracted with methylene chloride. The chromatographic conditions used easily separate the MEAA ethyl ester from interferences within mouse urine. The application of this procedure to urine samples collected from mice demonstrated that MEAA was detectable after oral (2000 mg/kg) or dermal (50 u L) exposure for 7 days to JP-8 at levels as high as 8.5 or 6.5 u g/mL, respectively. This pilot demonstration indicated that total urinary MEAA was a viable biomarker for the two routes of JP-8 exposure in laboratory mice.
Acetic-acids; Acids; Laboratory-animals; Animals; Animal-studies; Jet-engine-fuels; Urinalysis; Gas-chromatography; Exposure-levels; Exposure-assessment; Biomarkers; Sampling;
Author Keywords: GC-MS; Glycol Ethers; Jet Fuel; JP-8; 2-Methoxyethoxy acetic acid; MEAA
U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health; Division of Applied Research and Technology, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, Ohio, 45226
DART; OD; HELD
Research Tools and Approaches: Exposure Assessment Methods
Toxicology Mechanisms and Methods