Arsenite modulation of the integrity of actin filaments and the increase in cell motility may be mediated through CDC42.
Qian-Y; Zhang-Z; Flynn-DC; Shi-X
FASEB J 2002 Mar; 16(4)(Part I):A171
Arsenite is a human carcinogen, however, the molecular mechanisms involved are poorly understood. By stimulating human endothelial cells (SVEC4-10) with arsenite, we found that arsenite modulated the integrity of actin filaments, and stimulated the formation of cell motility organelle structures within 5 minutes. Further, we found arsenite increased the cell motility, indicating that structural changes in cell morphology induced by arsenite are relevant to cellular functions. Affinity absorption assays with GST-PAK-PBD demonstrated that arsenite activated small GTPase CDC42 and Rac. We also observed that arsenite induced the formation of hygrogen radicals in SVEC4-10 cells, which are known to exert a regulatory role in arsenite induced effects on SVEC4-10 cells. Taken together, our data demonstrate that arsenite increases cell motility, which may be involved in cancer matastasis.
Arsenites; Carcinogens; Carcinogenicity; Carcinogenesis; Cell-morphology
PPRB, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
Conference/Symposia Proceedings; Abstract
Research Tools and Approaches: Cancer Research Methods
The FASEB Journal