Bio-calorimeter system to measure the metabolic rate of laboratory animals after inhalation exposure to toxic materials.
Goldsmith-WT; McKinney-WG; Huffman-JS; Fedan-JS; Reynolds-JS; Goldsmith-E; Smith-J; Frazer-DG
Am J Respir Crit Care Med 1999 Mar; 159(3)(Suppl):A490
Metabolic rate of animals can be affected by a variety of toxic agents. Virtual instrument software was developed to capture and display data provided by a modified direct calorimeter. The calorimeter system was evaluated by examining four groups of rats (n=6) injected with thyroxine(T4) or solvent alone. Half of each group was exposed to ozone (2 ppm for 3 hours) or air. Breathing rates in addition to metabolic rates were measured pre-exposure, immediately post-exposure and 18 hours post-exposure. Metabolic rates were computed following equilibration which occurred in ten to twenty minutes. The calorimeter system provides non-invasive, reproducible results which can be used to evaluate an animal's metabolic response to toxic materials. In addition, the system displays and saves the data in real time and is easily operated.
Analytical-processes; Analytical-methods; Animal-studies; Animals; Laboratory-animals; Exposure-assessment; Metabolic-rate; Computer-software
Abstract; Conference/Symposia Proceedings
American Journal of Respiratory and Critical Care Medicine