NIOSHTIC-2 Publications Search
Oxidative stress and cytotoxicity of phenol and AMVN in human keratinocytes: evidence for enhanced lipid and thiol oxidation and antioxidant depletion.
Shvedova-AA; Kommineni-C; Tyurina-YY; Tyurin-VA; Castranova-V; Kagan-VE
Proceedings of the Advances in the Biology and Treatment of the Skin Symposium, Piscataway, NJ, June 23-25, 1999. Piscataway, NJ: Environmental and Occupational Health Sciences Institute, 1999 Jun; :1
A variety of phenolic compounds that are utilized in industry (e.g., for production of phenol-formaldehyde resins, paints and lacquers, cosmetics and pharmaceuticals) are toxic to skin (i.e., may cause rashy dermal inflammation, contact dermatitis, leucoderma, or cancer promotion). The biochemical mechanisms of cytotoxicity of phenolic compounds are not well understood. We hypothesized that enzymatic one electron oxidation of phenolic compounds resulting in generation of phenoxyl radicals may be an important contributor to their cytotoxic effects. Phenoxyl radicals are readily reduced (regenerated) by thiols, ascorbate and other intracellular reductants (e.g., NADH or NADPH). Hence, phenolic compounds may undergo enzymatically-driven redox-cycling, thus causing oxidative stress. To experimentally test the hypothesis, we performed measurements of thiols, lipid peroxidation and total antioxidant reserves in normal human keratinocytes incubated with phenol and compared the effects to those produced by an azo-initiator of peroxyl radicals, AMVN, 2,2' -azobis(2,4-dimethylvaleronitrile). Using a newly developed cis-parinaric acid-based procedure to assay site specific oxidative stress in membrane phospholipids, we found that phenol at subtoxic concentrations. (50 microM and 500 microM) caused oxidation of phosphatidylcholine and phosphatidylethanolamine (but not of phosphatidylserine) in keratinocytes. The magnitude of the phenol-induced changes in phospholipids was quantitatively comparable to those induced by AMVN. Measurements with ThioGlo showed that phenol dramatically depleted GSH. Luminol-enhanced chemiluminescence assay demonstrated a significant decrease in total antioxidant reserves of keratinocytes exposed to phenol. Incubation of ascorbate-preloaded keratinocytes with phenol produced an EPR-detectable signal of ascorbate radicals. We conclude that redoxcycling of the one-electron oxidation product of phenol, its phenoxylradical, is involved in the oxidative cytotoxic effects.
Cytotoxins; Cytotoxic-effects; Cytotoxicity; Phenolic-compounds; Phenols; Contact-dermatitis; Contact-allergies; Dermatitis; Allergic-dermatitis; Allergic-reactions; Analytical-processes; Analytical-chemistry; Statistical-analysis
Abstract; Conference/Symposia Proceedings
Proceedings of the Advances in the Biology and Treatment of the Skin Symposium, Piscataway, NJ, June 23-25, 1999
PA; NJ; WV
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