Murine immune responses to natural rubber latex proteins.
Woolhiser-MR; Munson-AE; Meade-BJ
Toxicologist 1999 Mar; 48(1-S):314
It has been estimated that up to 17% of the 5.5 million U.S. health care workers (HCW) demonstrate IgE antibodies to natural robber latex (NRL) products; an even higher prevalence of latex allergic spina bifida patients have been reported (as high as 700A.). Furthermore, different allergen specific IgE profiles have been associated with HCW as compared to spina bifida patients. Although the exposure routes which elicit allergic responses are identifiable, the route(s) by which NRL sensitization occurs remains unclear. Our objective is to develop murine models representative of different routes of exposure to better understand latex sensitization and for testing intervention methodologies. Female BALB/c mice were treated for 2 months subcutaneously (sc) or topically (abraded or intact skin sites) with latex proteins ranging from 1.5g -200Microg per exposure. Following sc exposure, total IgE levels were elevated in a dose response fashion beginning at day 14 and peaked near 12,000ng/ml following 8 weekly injections of 200microg. Likewise, IgE levels were increased 9-fold after two weeks of daily applications to abraded skin sites using 150microg of latex protein diluted in acetone. Fifty microg applications to abraded skin sites also resulted in greater than a 4 fold elevation in total IgE by day 16 whereas intact skin exposure produced no increases until day 37. By day 53, total IgE concentrations following SOll8 topical exposures were comparable between "abraded" and "intact" mice. Antigen specificity was demonstrated when in vitro stimulation of splenocytes (sc exposed mice) with 2 degrees J.1g/ml latex: proteins caused a 500% increase in3H-thymidine uptake. Immunoblots identified IgE specific for all of the major latex allergens (Hev b1- 7) following either sc or topical latex exposure. Functional latex specific IgE was demonstrated in a murine Active Cutaneous Anaphylaxis assay within 5 minutes of intradermal latex protein challenge (approximately 10g) in the ear. These results indicate that BALB/c mice should serve as an acceptable model to study hypersensitivity to NRL.
Toxins; Toxicology; Toxic-materials; Laboratory-animals; Animal-studies; Statistical-analysis; Analytical-chemistry; Analytical-methods; Chemical-analysis; Dose-response; Exposure-assessment; Lymph-nodes; Ears; Irritants; Allergic-reactions; Allergens; Allergic-disorders; Allergies; Health-care-personnel; Health-care; Sensitivity-testing; Sensitization
The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana