Cytokine and growth factor gene expression in response to trimethyl tin (TMT)-induced neurotoxicity in the adult rat hippocampus.
Toxicologist 1999 Mar; 48(1-S):239
Cytokines and growth factors are proposed to play a role in the homeostasis of the central nervous system (CNS) and its response to injury and disease. A role for these pleiotropic mediators in the early activation of microgial and astrocytic reactions to neural injury constitutes a dominant theme in contemporary neuroscience literature, despite the fact that most data are derived from in vitro preparations. Understanding the earliest events associated with toxicant-induced brain injury is necessary to detect and modify CNS damage following injury or disease. Here we examined the in vivo expression of interleukin-1 alpha (IL-1alpha), interleukin-6 (IL-6), transforming growth factor-beta (TGF-beta), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) using the TMT model of neuronal injury (8mg/kg i.p.) using semi-quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) at 24 hours post TMT. This timepoint was chosen based on reports in the literature that mRNA for TGF-beta and IL-6 are up-regulated at this time with other types of nerve injury. No significant changes for BDNF, NGF, or IL-1alpha were seen. mRNA for TGF-beta and IL-6 were increased slightly less than 2 fold, which is a very small increase, but is consistent with published reports. These results suggest that these neuroimmune/neurotrophic mediators do not play a crucial role in the activation of microglia and astrocytes following toxicant-induced injury to the CNS. Additional toxicants need to be examined in order to determine the generality of these observations to the neurotoxic condition.
Toxicology; Toxins; Toxic-materials; Statistical-analysis; Analytical-methods; Analytical-chemistry; Chemical-analysis; Animal-studies; Laboratory-animals; Central-nervous-system; Growth-factors; Nervous-system; Neurological-system; Neurotoxicology; Neurotoxins; Neurotoxicity; Neurotoxic-effects; Nerve-damage
The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana