Persistant pulmonary inflammation after intratracheal instillation of abrasive blasting agents.
Minhas-NS; Battelli-LA; Porter-DW; Goldsmith-WT; Dotson-A; Jones-W; Greskevitch-M; Ma-JYC; Hubbs-AF
Toxicologist 1999 Mar; 48(1-S):51
Use of silica sand in abrasive blasting is associated with pulmonary disability due to silicosis. However, the pulmonary toxicity of substitutes for silica sand in abrasive blasting remains incompletely investigated. Therefore, Sprague Dawley rats received a single intratracheal instillation (IT) dosage of 2.5 or 10 mg of the respirable fractions of abrasive blasting agents collected from the air during blasting of a steel bar. Four weeks post-IT, the right lung lobe was lavaged to isolate bronchoalveolar lavage cells and acellular bronchoalveolar lavage fluid (BALF); the left lung was processed for histopathology. Polymorphonuclear leukocyte (PMN) cell yield and alveolar macrophage (AM) chemiluminescence (CL) were significantly increased above control levels' in rats instilled with coal slag (2.5 and 10 mg), silica sand (10 mg), staurolite (10 mg),and treated sand (10 mg). Rats which received garnet (10 mg) also had increased PMN numbers versus control but AM CL was not increased. Lactate dehydrogenase (LDH) was significantly elevated in the BALF of rats instilled with coal slag (2.5 and 10 mg), garnet (2.5 and 10 mg), silica sand (2.5 and 10 mg), staurolite (10.0 mg), and treated sand (10 mg), whereas BALF albumin was not increased in any of the exposed rats versus control. Hydroxyproline was significantly elevated over saline controls only in the rats receiving coal slag (10 mg). However, trichromestained lung sections showed focal to multifocal, minimal fibrosis in 6 of 6 silica exposed rats (10 mg) and 3 of 6 coal slag exposed rats (2.5 and 10 mg). Only specular hematite (iron oxide) failed to increase some measure of pulmonary toxicity.
Animal-studies; Laboratory-animals; Statistical-analysis; Analytical-methods; Analytical-chemistry; Exposure-assessment; Toxicology; Toxins; Toxic-materials; Sand-blasting; Sand-blasters; Silica-dusts; Silicates; Silicosis; Pulmonary-system-disorders; Pulmonary-disorders; Respiratory-system-disorders; Respiratory-irritants; Abrasive-blasting; Lung-cells; Lung-disorders; Lung; Lung-irritants
The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana