Two dimensional electrophoretic protein profile associated with EMF inhibition of cytostatic effect of tamoxifen on MCF-7 cell growth.
Kanitz-MH; Afzal-SMJ; Harland-J; Liburdy-RP; Savage-RE
Bioelectromagnetics Society (BEMS) Abstract Book, Twentieth Annual Meeting, June 7-11, 1998, St. Pete Beach, Florida. Frederick, MD: Bioelectromagnetics Society, 1998 Jun; :237
Epidemiological studies suggest an increased risk of breast cancer is associated with exposure to electromagnetic fields (EMFs). Previous investigations have shown that a 12 mG, 60 Hz magnetic field significantly inhibits the cytostatic action of pharmacological doses of tamoxifen (10-7 M) on the growth of MCF-7 human breast cancer cells. Here we use high resolution two dimensional polyarylamide gel electrophoresis (2D PAGE) to investigate phenotypic biochemical alterations which may represent protein biomarkers of effect of exposure of drug-treated human breast cancer cells to an environmental-level magnetic field. Solubilized MCF-7 cell fractions (20 ug) from each treatment group (control - 2 mG field; control - 12 mG field; tamoxifen - 2 mG field; tamoxifen - 12 mG field) are subjected to 2D PAGE using the Anderson ISODALT method. Computer analysis and comparison of gel patterns across treatment groups were performed using PDQUEST software (BioRad Labs, CA). The protein alterations associated with the observed 12 mG blocking effect of tamoxifen inhibition are characterized with respect to Mr and pI. These studies may provide an understanding at the biochemical level of the EMF-induced reversal of tamoxifen's cell growth inhibition of human breast cancer cells and offer some insight into the putative role of EMF in human breast cancer risk.
Cell-growth; Epidemiology; Electromagnetic-fields; Risk-factors; Risk-analysis; Exposure-levels; Breast-cancer; Cancer; Biomarkers
Bioelectromagnetics Society (BEMS) Abstract Book, Twentieth Annual Meeting