Adverse responses to semisynthetic metal working fluids in B6C3F1 mice.
Al-Humadi-NH; Kommineni-C; Shvedova-AA; Battelli-L; Castranova-V
Toxicologist 1999 Mar; 48(1-S):76
About 10 million industrial workers of both sexes are exposed to metal working fluids (MWFs) via inhalation or skin or both. Our preliminary results, following dermal app1ication of 200microl of 50 % unused (neat) semi-synthetic MWF (pH 7 or pH 9.7) to the unshaved backs of 6 week old B6C3F1 mice twice a week for 6 weeks., show significant increase in weights of the liver of both sexes. The present study is to determine if this change is related to oxidative stress consequent to .MWF exposure. Therefore we exposed 6 month old mice of both sexes to MWFs following the protocol except that the topical application was with 5% MWFs, 5 days a week for 13 weeks. Chemical analyses of tissues from these mice indicate significant reduction in ascorbic acid and glutathione levels in the liver of both sexes and in the gonads of the male. Malonaldebyde levels in male liver and gonads show significant increase. The skin histamine level significantly increased in the female. These results suggest that are absorbed through the skin, exhibit liver toxicity, and may represent an important health risk to industrial workers exposed to these fluids.
Metal-industry-workers; Metal-industry; Metal-refining; Metal-workers; Metalworking-fluids; Metalworking-industry; Metalworking; Animal-studies; Animals; Laboratory-animals; Statistical-analysis; Liver; Chemical-analysis; Analytical-chemistry; Analytical-methods
The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana