NIOSHTIC-2 Publications Search

DNA-sequence determination of exon 2 of a novel HLA-DPB1 allele, HLA-DPB1*0403.

Authors
Weston-A; Ensey-JS; Frye-BL
Source
DNA Seq 2005 Jun; 16(3):235-236
NIOSHTIC No.
20027632
Abstract
Sequence determination using HLA-DPB1 allele-specific primers for a DNA sample donated by an African-American individual revealed the presence of a novel haplotype. This new allele was found as a heterozygote together with HLA-DPB1*0402. The new allele was similar to HLA-DPB1*1601, however, it varied in two single nucleotide polymorphisms resulting in alanine residues at positions 55 and 56 of the mature protein rather than aspartic acid and glutamic acid, respectively. Allele-specific DNA-sequence determination was verified by sequence determination in forward and reverse directions after cloning in pCR2.1. This cloning strategy resulted in DNA products representing 19 clones confirming the novel allele (GenBank accession number AY823995 and is now listed in the IMGT/HLA database as HLA-DPB1*0403) and 17 clones representing HLA-DPB1*0402.
Keywords
Demographic-characteristics; Racial-factors; Sampling; Nucleotides; DNA-adducts; Gene-mutation; Genetic-factors; Genetics; Molecular-biology; Molecular-structure
Contact
A. Weston, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505-2888, USA
CODEN
DNSEES
Publication Date
20050601
Document Type
Journal Article
Email Address
agw8@cdc.gov
Fiscal Year
2005
Issue of Publication
3
ISSN
1042-5179
NIOSH Division
HELD
Source Name
DNA Sequence
State
WV
Page last reviewed: April 12, 2019
Content source: National Institute for Occupational Safety and Health Education and Information Division