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DNA-sequence determination of exon 2 of a novel HLA-DPB1 allele, HLA-DPB1*0403.

Weston-A; Ensey-JS; Frye-BL

DNA Seq 2005 Jun; 16(3):235-236

http://dx.doi.org/10.1080/10425170500061442

20027632

Sequence determination using HLA-DPB1 allele-specific primers for a DNA sample donated by an African-American individual revealed the presence of a novel haplotype. This new allele was found as a heterozygote together with HLA-DPB1*0402. The new allele was similar to HLA-DPB1*1601, however, it varied in two single nucleotide polymorphisms resulting in alanine residues at positions 55 and 56 of the mature protein rather than aspartic acid and glutamic acid, respectively. Allele-specific DNA-sequence determination was verified by sequence determination in forward and reverse directions after cloning in pCR2.1. This cloning strategy resulted in DNA products representing 19 clones confirming the novel allele (GenBank accession number AY823995 and is now listed in the IMGT/HLA database as HLA-DPB1*0403) and 17 clones representing HLA-DPB1*0402.

Demographic-characteristics; Racial-factors; Sampling; Nucleotides; DNA-adducts; Gene-mutation; Genetic-factors; Genetics; Molecular-biology; Molecular-structure

A. Weston, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505-2888, USA

DNSEES

20050601

Journal Article

agw8@cdc.gov

2005

3

1042-5179

HELD

DNA Sequence

WV