Comparative toxicity of nanomaterials in vitro and in vivo.
Shvedova-AA; Murray-AR; Johnson-VJ; Gorelik-O; Arepalli-S; Hubbs-A; Mercer-RR; Baron-P; Maynard-AD; Kagan-VE; Potapovich-AI; Castranova-V; Kisin-E
Abstr Pap - Am Chem Soc 2005 Mar; 229(Part 1):014-IEC
Society is currently amidst with revolutionary developments of remarkable new technologies based on novel applications of nanomaterials. A comparative in vitro study of nanosized particles revealed dose-dependent formation of granulomatous bronochointerstitial pneumonia, fibrosis, and changed pulmonary function in C57BL/6 mice. Administration of CNT to mice also resulted in a dose-dependent augmentation of biomarkers of inflammation quantified by lavage cell counts, total protein, lactate dehydrogenase and glutamyltranspeptidase activities in BAL fluids and accumulation of pro-inflammatory and pro-fibrotic cytokines. Overall, our data suggest that in vivo exposure to CNT leads to pulmonary toxicity realized through the synergized interactions of inflammatory response and oxidative stress culminating in the development of multifocal granulomatous pneumonia and fibrosis. The value of both approaches will be discussed in line with assessment of adverse outcomes of nanomaterials.
In-vitro-studies; In-vivo-studies; Cytotoxins; Cytotoxic-effects; Cytotoxicity; Laboratory-animals; Animals; Animal-studies; Exposure-levels; Exposure-assessment; Fibrosis; Biomarkers; Pulmonary-system-disorders; Respiratory-system-disorders; Nanotechnology
Abstract; Conference/Symposia Proceedings
HELD; OD; DART
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Abstracts of papers - American Chemical Society