Comparison of three methods for determining removal of stratum corneum using adhesive tape strips.
Proceedings of the International Conference on Occupational and Environmental Exposures of Skin to Chemicals: Science & Policy, Arlington, VA, September 8-11, 2002. Morgantown, WV: National Institute for Occupational Safety and Health, 2002 Sep; :1
Adhesive tape stripping (ATS) has been used to remove layers of the outermost stratum corneum from skin. These tapes can be used to measure the physical condition of the skin or for quantifying exogenous or endogenous compounds present within the skin. There is also the need for a non-destructive approach to measure the mass of stratum corneum adhering to the tape so that samples taken for chemical analysis can be normalized per the amount of stratum corneum present. Although used for years, there is still not a uniform approach for performing ATS sampling and several potentially influential variables have not been carefully evaluated. Our goal here is to evaluate a new instrumental procedure that might provide us the means of performing both of the above measurements of ATS on samples collected from workers in various occupations. We report our analysis of ATS samples using three types of analysis: (1) protein assay of keratin mass, (2) desquamation index (CuDerm, Inc, Dallas TX), and (3) an instrumental light reflective device for direct measurement of adhering skin (Visioscan VC 98, Courage & Khazaka, Koln, Germany). Only the latter method has the ability to both assess skin condition and possibly mass of stratum corneum on the ATS samples. Measurement analysis using the above approaches was performed on 90 skin samples and 10 blank samples.
Skin-tests; Skin; Chemical-analysis; Analytical-methods; Skin-absorption; Skin-exposure; Analytical-processes; Analytical-chemistry
Proceedings of the International Conference on Occupational and Environmental Exposures of Skin to Chemicals: Science & Policy, Arlington, VA, September 8-11, 2002