Improved method to measure alkoxyacetic acid in urine: a solid phase extraction - gas chromotography - mass spectrometry method for 2-butoxyacetic acid (the human metabolite of 2-butoxyethanol), 2-[(2H9)butoxy]acetic acid, and 2-(3-methylbutoxy)acetic acid.
Brown-KK; Cheever-KL; Butler-MA; Shaw-PB; McLaurin-JL
Proceedings of the International Conference on Occupational and Environmental Exposures of Skin to Chemicals: Science & Policy, Arlington, VA, September 8-11, 2002. Morgantown, WV: National Institute for Occupational Safety and Health, 2002 Sep; :1-7
Historically, the analysis of polar alkoxyacetic acid metabolites has been problematic, involving laborious extraction and derivatization (e.g., Smallwood et al., 1988. Appl. Ind. Hyg. 3(2), 47-50). Thus, NIOSH researchers developed a method to biomonitor alkoxyacetic acids, based on a published method for butoxyacetic acid (Shih et al., 1999. J. Occup. Environ. Med. 56(7), 460-467). Shih et al. had eliminated the derivatization step and its imprecision contribution in measuring butoxyacetic acid by using a free fatty acid phase (FFAP) capillary column in the gas chromatography/mass spectrometry (GC/MS) system. In addition, the NIOSH method eliminated the need for the laborious liquid extraction by replacing it with solid phase extraction (SPE), using a pH-resilient anion exchange resin. This improved method was then optimized for the measurement of butoxyacetic acid (BAA), using novel deuterated butoxyacetic acid (dBAA) as an isotopically diluted internal standard and novel 2-methylbutoxyacetic acid (MBAA) as a recovery/instrumental performance standard.
Chemical-properties; Chemical-processing; Urinalysis; Urine-chemistry; Biomarkers; Metabolites; Exposure-assessment; Chromatographic-analysis; Analytical-chemistry; Mass-spectrometry; Analytical-processes; Analytical-methods
Proceedings of the International Conference on Occupational and Environmental Exposures of Skin to Chemicals: Science & Policy, Arlington, VA, September 8-11, 2002