Development of a procedure for the quantification of the biomarker (2-methoxyethoxy)acetic acid in human urine.
B'Hymer-C; Butler-MA; Cheever-KL
Proceedings of the International Conference on Occupational and Environmental Exposures of Skin to Chemicals: Science & Policy, Arlington, VA, September 8-11, 2002. Morgantown, WV: National Institute for Occupational Safety and Health, 2002 Sep; :1
A simple and effective procedure was developed for the detection and quantification of (2-methoxyethoxy)acetic acid (MEAA), which is a metabolite and biomarker for exposure to 2-(2-methoxyethoxy)ethanol. Human dermal exposure to 2-(2-methoxyethoxy)ethanol is of concern because of the general toxicity of glycol ethers and this compound, specifically, is used as an anti-icing additive to the military jet fuel, JP-8. Possible dermal absorption by aircraft fuel cell maintenance personnel is of concern; therefore, a test procedure for MEAA in urine samples was devised. The urine sample preparation consisted of ethyl acetate extraction, followed by esterification of the MEAA to produce the ethyl ester. Extraction of the ethyl ester with methylene chloride and concentration of sample solution to a one milliliter volume was done to produce the final solution for analysis. Measurement was by a gas chromatograph equipped with a mass selective detector (MSD) using a 50-m X 0.20-mm (id) HP-1 capillary column and a temperature program of 50 degrees to 230 degrees-C. Deuterated (2-butoxy)acetic acid was used as a procedural internal standard for this analysis procedure. Ion m/z 59 was monitored for the ester of MEAA and ion m/z 66 was monitored for the internal standard. A recovery study of spiked urine demonstrated good accuracy and precision; recovery varied between 95-103% for 2 to 20 micrograms/ml MEAA spiked urine samples. The limit of detection (LOD) was determined to be approximately 0.1 micrograms/ml for this procedure.
Chemical-properties; Chemical-processing; Urinalysis; Urine-chemistry; Biomarkers; Metabolites; Exposure-assessment; Chromatographic-analysis; Analytical-chemistry; Analytical-methods; Analytical-processes
Proceedings of the International Conference on Occupational and Environmental Exposures of Skin to Chemicals: Science & Policy, Arlington, VA, September 8-11, 2002