Lack of association between antioxidant gene polymorphisms and progressive massive fibrosis in coal miners.
Yucesoy-B; Johnson-VJ; Kashon-ML; Fluharty-K; Vallyathan-V; Luster-MI
Thorax 2005 Jun; 60(6):492-495
Oxidative stress plays a major role in the pathogenesis of interstitial lung diseases. The antioxidant enzymes glutathione S-transferases (GST) and manganese superoxide dismutase (MnSOD) are important components of lung defence against oxidative stress, and polymorphisms in the genes which regulate their expression may represent important disease modifiers. A matched case-control study was conducted to determine the influence of the GSTP1, GSTT1 and MnSOD polymorphisms on susceptibility to progressive massive fibrosis (PMF). Seven hundred ex-coal miners were included in the study; 350 were classified as PMF cases while 350 with a similar underground mining tenure but no clinical or histological evidence of lung disease served as controls. Genotype analysis was performed on genomic DNA, using a 5' nuclease PCR assay. None of the individual investigated polymorphisms and two-way gene-gene interactions had a statistically significant association with PMF. The results of this study suggest that polymorphic genotypes within the GST gene cluster and MnSOD do not affect individual susceptibility to PMF.
Genetic-factors; Genes; Antioxidants; Fibrosis; Coal-miners; Pneumoconiosis; Dust-exposure; Diseases; Lung-disease; Pulmonary-system-disorders; Respiratory-system-disorders; Mineral-dusts; Lung-tissue; Environmental-exposure; Pathogenesis; Fibrogenesis; Fibrogenicity
B. Yucesoy, PhD, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, West Virginia 26505-2888