Changes in gene and protein expression in magnetic field-treated human glioma cells.
Savage RE; Kanitz MH; Lotz WG; Conover D; Hennessey EM; Hanneman WH; Witzmann FA
Toxicol Mech Methods 2005 Mar; 15(2):115-120
Because few cancer studies have examined protein profiles and genetic regulation from a single carcinogen exposure, the objective of this study was to determine genetic change via microarray and to evaluate whether that change was a precursor to cellular protein changes. In separate but experimentally identical studies, human glioma SF767 cells were exposed for 3 h to 60-Hz magnetic fields (sham or 1.2 microT). Microarray results suggested that magnetic field treatment resulted in the up-regulation of 5 genes, whereas 25 genes were down-regulated. The mean abundance of 10 identified proteins was altered following 1.2 microT exposure relative to sham (3 increase, 7 decrease). These studies suggest a limited but complicated response in the glioma cells to the magnetic field treatment.
Genes; Proteins; Genetic-factors; Exposure-levels; Carcinogens; Carcinogenicity; Carcinogenesis;
Author Keywords: Genomics; Glioma; Magnetic Field; Proteomics
Russell E. Savage Jr., NRS, EPHB, DART, NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226-1998
Research Tools and Approaches: Control Technology and Personal Protective Equipment
Toxicology Mechanisms and Methods