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A headspace procedure for the quantification of 1- and 2-bromopropane in human urine.

B'Hymer C; Cheever KL
Toxicologist 2005 Mar; 84(Suppl 1):40
A test procedure was developed to detect and quantify the levels of 1- and 2-bromopropane in urine. 1-Bromopropane (1-BP) is a commonly used industrial solvent, and 2-bromopropane (2-BP) is often found as an impurity component in industrial grade 1-BP. Both are of a health concern for exposed workers because of their chronic toxicity. Central neurological and peripheral neuropathy disorders have been reported in workers exposed to 1-BP; reproductive and hematopoietic disorders has been reported for workers exposed to 2-BP. In the test procedure described, urine samples were diluted with deionized water and placed into a sealed headspace vial. A static-headspace sampler (Teledyne-Tekmar Model 7000) was used to heat each sample at 75oC for a 35 minute equilibrium time. Quantification of the two analytes was by means of a gas chromatograph equipped with a dimethylpolysiloxane capillary column and an electron capture detector. 1-Bromobutane was used as an internal standard for this test procedure. A multi-level recovery study using fortified urine samples (0.5 to 8 µg/ml) demonstrated full recovery; 104 to 121% recovery was obtained. Precision ranged from 5 to 17 % for the 15 to 20 spiked samples at each level analyzed over multiple experimental trial days. The limit of detection or this test procedure was approximately 2 ng/ml 1-BP and 7 ng/ml 2-BP in urine. A six week storage study was conducted to determine the most appropriate method to collect and store fortified urine samples. Glass serum vials with crimped caps and Teflon lined septa stored at 4oC demonstrated full recovery of both 1-BP and 2-BP.
Urinalysis; Solvents; Health-hazards; Workers; Worker-health; Occupational-exposure; Exposure-levels; Toxic-effects; Toxins; Neurological-diseases; Neurological-reactions; Neurological-system; Neuropathy
Publication Date
Document Type
Fiscal Year
NIOSH Division
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
The Toxicologist. Society of Toxicology 44th Annual Meeting and ToxExpo, March 6-10, 2005, New Orleans, Louisiana
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division