A method for the simultaneous measurement of specific IgGs to five CDC select bioterrorism agents in serum.
Biagini RE; Sammons DL; Smith JP; MacKenzie BA; Striley CA; Robertson SA; Snawder JE; Quinn CP
Toxicologist 2005 Mar; 84(Suppl 1):158
The Centers for Disease Control and Prevention has classified several agents which may be used in a bioterrorism attack as select agents. They are classified as such based on ease of dissemination, mortality/morbidity rate, and potential for social disruption. A subset of these agents include Bacillus anthracis (Ba), Yersinia pestis (Yp), Francisella tularensis (Ft), ricin toxin (RT) and staphylococcus enterotoxin B (SEB). Exposure/infection with these agents has been shown to cause the production of specific serum IgG antibodies. Comparison of pre- and post-incident IgG antibody levels has been shown to be a useful method to biologically monitor decontamination and clean-up workers for potential exposure to bioterrorism agents (Occ Environ Med. 61:703-708, 2004). We describe a fluorescent covalent microsphere immunoassay (FCMIA) to measure specific IgG antibodies to Ba (protective antigen [PA] and lethal factor [LF]), Yp (F1 and V antigens), Ft, RT and SEB simultaneously in human Ba vaccinee sera which had been fortified with animal select agent specific IgG antibodies. Intra- and interassay coefficients of variation were <15% (N=3). There were no significant differences (P>0.70) in dilution curves when the assays were performed individually vs. multiplexed. When the observed vs. expected dilution curves were compared, highly linear relationships were observed (mean r2=0.985 +/- 0.006 [SD], P<0.001). Finally, the curves yielded linear responses for most analytes upon serum dilution from 1 x 10^2 to 1 x 10^5. These data indicate that multiplexed FCMIA is a method to measure specifics IgGs in serum to CDC select agents and may be of value in screening either decontamination workers or the general population for exposures/infections to these agents.
Biological-warfare-agents; Mortality-rates; Mortality-data; Morbidity-rates; Exposure-levels; Biological-agents; Biological-weapons; Infection-control; Biological-monitoring; Decontamination; Occupational-exposure; Occupational-hazards; Workers
The Toxicologist. Society of Toxicology 44th Annual Meeting and ToxExpo, March 6-10, 2005, New Orleans, Louisiana