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Free radical scavenging, DNA protection, and inhibition of lipid peroxidation mediated by uric acid.

Stinefelt B; Leonard SS; Blemings KP; Shi X; Klandorf H

Ann Clin Lab Sci 2005 Jan; 35(1):37-45

http://www.annclinlabsci.org/cgi/content/abstract/35/1/37

20026395

Uric acid (UA) has been proposed to be the dominant antioxidant in birds. The objective of this study was to investigate the quenching effect of varying concentrations of UA, including those found in avian plasma, on specific reactive oxygen species (ROS) and to determine the ability of UA to protect DNA and cellular membranes from ROS-mediated damage. Hydroxyl (OH) and superoxide (O2) radicals were detected by electron spin resonance (ESR) and their presence was reduced following addition of UA (p <0.05) in a concentration-dependent manner. UA inhibited hydroxyl-mediated DNA damage, indicated by the presence of more precise, dense bands of Hind III DNA after agarose gel electrophoresis and ethidium bromide staining (p <0.05). Lipid peroxidation of silica-exposed RAW 264.7 cell membranes was diminished (p <0.02) after addition of UA to the cell incubation mixture. These studies demonstrate that UA scavenges hydroxyl and superoxide radicals and protects against DNA damage and lipid peroxidation. These results indicate specific antioxidant protection that UA may afford birds against ROS-mediated damage.

Free-radicals; Free-radical-generation; Lipids; Lipid-peroxidation; Antioxidants; DNA-damage; Hydroxyl-groups; Silica-dusts; Silicates; Exposure-levels; Author Keywords: uric acid; oxidative stress; electron spin resonance; lipid peroxidation; DNA oxidation

Kenneth P. Blemings, Ph.D., West Virginia University, P.O. Box 6108, Morgantown, WV 26506, USA

ACLSCP

20050101

Journal Article

kbleming@wvu.edu

2005

1

0091-7370

HELD

Research Tools and Approaches: Cancer Research Methods

Annals of Clinical and Laboratory Science

WV