Effect of age, diet restriction and aminoguanidine on pentosidine in the skin of broiler breeder hens.
Igbal-M; Probert-LL; Klandorf-H; Van Dyke-K; Al-humadi-NH
FASEB J 1998 Mar; 12(5)(2)(S):A879
Non-enigmatic glycosylation contributes to the formation of cross-links which leads to the deterioration in the structure and function of tissue protein. Whether the elevated concentrations of plasma glucose in chickens accelerates the accumulation of cross-links has not been determined. The objectives of this study were to determine the effect of diet restriction (DR) and the cross-linking inhibitor aminoguandine (AG) on the rate of accumulation of the glycoxidation product, pentosidine, in the skin of naturally hyperglycemuc broiler breeder (BB) hens. BB female chicks (ne=450) were randomly assigned to four groups from 8 to 125 wks after hatch. ad libitum (AL), diet restricted (DR); ad libilum and diet restricted groups supplemented with 400 ppm AG each (AL + AG and DR + AG respectively). Skin pentosidine was isolated by HPLC and oxidative stress was measured by luminometery. Results showed that the accumulation of pentosidine increased linearly (P<0.001) with age. DR significantly (P<0.001) retarded the rate of accumulation of pentosidine. The concentration of pentosidine in AL + AG group was comparable with that of DR group and skin pentosidine was lowest (P<0.001) in both DR and DR + AG groups as compared to AL and AL + AG groups. It was concluded that the rate of accumulation of pentosidine can be retarded by DR and AG. In addition, the data supports the view that pentosidine can be used as a biomarker for the study of aging in chickens.
Age-factors; Diet; Dietary-effects; Skin-exposure; Animals; Animal-studies; Tissue-disorders; Biomarkers; Laboratory-animals; Genotoxic-effects
Abstract; Conference/Symposia Proceedings
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