Cytotoxic and genotoxic effects of single wall carbon nanotube exposure on human keratinocytes and bronchial epithelial cells.
Shvedova-AA; Kisin-E; Keshava-N; Murray-AR; Gorelik-O; Arepalli-S; Gandelsman-VZ; Castranova-V
Abstr Pap - Am Chem Soc 2004 Mar; 227(Part 1):IEC-20
Carbon nanotubes are new members of carbon allotropes similar to fullerenes and graphite. Because of their unique electrical, mechanical and thermal properties, carbon nanotubes are being evaluated for novel applications in electronics, aerospace and computer industries. Occupational exposure to graphite and carbon materials has been associated with increased incidence of skin and lung diseases, e.g.. carbon fiber dermatitis, hyperkeratosis, naevl, asthma, pneumoconiosis and cancer. However, the potential toxicity and mechanisms of action of single wall carbon nanotubes (SWCNT) have yet to be determined. The present talk will. be focused on adverse effects of SWCNT studied in cell culture models - human epidermal keratinocytes (HaCaT) and bronchial epithelial cells (BEAS-2B). SWCNT caused ultra-structural and morphological changes, loss of cell integrity and apoptosia. accelerated oxidative stress indicated by the formation of carbon-centered and hydroxyl radicals, accumulation of peroxidative products, and depletion of antioxidants in the cells. Transcriptional response to SWCNT in BEAS2 and HaCaT cells detected by microarray analysis revealed alterations of a number of genes including those involved in oxidative stress. The data suggest that exposure to SWCNT may lead to dermal and pulmonary toxicity, and identify oxidative stress as one of potentially important mechanisms of cell injury.
Cell-damage; Cell-alteration; Cell-biology; Cellular-function; Cellular-respiration; Cellular-reactions; Genes; Genotoxic-effects; Skin-disorders; Pulmonary-system-disorders; Respiratory-system-disorders; Antioxidants; Antioxidation; Cytotoxic-effects; Cytotoxicity; Cytotoxins; Nanotechnology
NIOSH, HELD, 1095 Willowdale Road, Morgantown, WV 26505
Abstract; Conference/Symposia Proceedings
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Abstracts of papers - American Chemical Society