Assessment of oxidative DNA damage in female workers exposed to perchloroethylene.
Toraason-M; Butler-MA; Ruder-A; Forrester-C; Taylor-L; Mathias-P; Wey-H
Toxicologist 2001 Mar; 60(1):86
Although perchloroethylene (PERC) has not been found to induce oxidative stress, the major metabolites (tri- and di-chloroacetic acids) have been reported to induce lipid peroxidation and oxidative DNA damage in rodents. In the present study, oxidative DNA damage and lipid peroxidation were assessed in 38 women with or without occupational exposure to PERC. 8-Hydroxydeoxyguanosine (8OHdG)/10^6 deoxyguanisine (dG) in leukocyte nuclear DNA was used as an index of steady-state oxidative DNA damage. Urinary 8-OHdG/g creatinine was used as an index of oxidative-DNA-damage repair. Urinary 8-epi-prostaglandin F2 (8-epiPGF)/g creatinine was used as an index of lipid peroxidation. The control and PERC exposed populations were separated into smokers and nonsmokers as tobacco smoking has been reported to increase oxidative DNA damage. Smoking status was not associated with significant differences in any of the indices of oxidative damage, although urinary 8-OHdG and 8-epi-PGF where higher in smokers than non smokers. PERC exposure was not associated with any significant differences in the urinary biomarkers of oxidative stress. In contrast, leukocyte 8-OHdG/dG was significantly reduced in both smoking and nonsmoking PERC exposed workers. In the absence of a difference in urinary DNA repair products, results suggest an association between PERC exposure and reduced leukocyte endogenous oxidative DNA damage.
Workers; Worker-health; DNA-damage; Demographic-characteristics; Sex-factors; Occupational-exposure; Laboratory-animals; Animals; Animal-studies; Tobacco-smoke; Smoking; Biomarkers
The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California