Development and evaluation of a rapid, sensitive, and specific immunochromatographic lateral flow assay for anthrax protective antigen immunity status.
Biagini-RE; Sammons-DL; Smith-JP; MacKenzie-BA; Striley-CAF; Robertson-SR; Snawder-JE
Am J Trop Med Hyg 2004 Oct; 71(Suppl 4):49
Evidence from animals suggests that anti-protective antigen (PA) igG from vaccination with Anthrax Vaccine Adsorbed (AVA) is protective against B. antrhacis infection. Measurement of anti-PA igG in human sera can be performed using their Enzyme-Linked Immunosorbent Assay (ELISA) or Fluorescent Covalent Microsphere Immunoassay, as reported previously. Both these methods are laboratory based. We describe the development of a rapid lateral flow immunochromatographic assay (LFIA) test kit for the measurement of anti-PA IgG in serum or whole blood (50 ul sample) using colloidal gold nanoparticles as the detection reagant and an internal control. Using sera from 19 AVA vaccines (anti-PA igG range, 2,4 - 267 ug/ml), 10 controls and PA-supplemented whole blood, we demonstrated the LFIA had a sensitivity of -2 ug/ml anti-PA IgG in sera and -14 ug/ml anti-PA IgG in whole blood. Pre-adsorption of sera with PA yielded negative anti-PA LFIAs. Internal controls were positive for all tests. The diagnostic sensitivity and specificity of the assay (for human sera) were 100% using FCMIA anti-PA IgG as standard. This kid has utility for determining the immune status of individuals (military personnel, decontamination workers) vaccinated with AVA or possibly exposed to sub-clinical levels of B. anthracis.
Antigens; Laboratory-animals; Animals; Animal-studies; Blood-samples; Blood-sampling; Vaccines; Nanotechnology
Abstract; Conference/Symposia Proceedings
The American Journal of Tropical Medicine and Hygiene. Abstracts Of The 53rd Annual Meeting Of The American Society Of Tropical Medicine And Hygiene: Fontainebleau Hilton, Miami Beach, Florida U.S.A., November 7-11, 2004