cDNA array analysis of the changes in gene expression specifically produced by neurotoxic doses of amphetamine: not quite mission impossible.
Bowyer-JF; Harris-A; Jakab-R; Delongchamp-R; Miller-D; O'Callaghan-J
Neurotoxicology 2004 Jun; 25(4):505
Dr. Bowyer presented the approach used in this collaborative study to examine the feasibility of using microarray technology to assess patterns of neurotoxicity. Changes in mRNA levels in specific cortical brain regions following neurotoxic doses of amphetamine were assessed using cDNA array (Clontech 1.2K Atlas cDNA array). Methods evaluation noted that the detection of a few selective two-fold changes involving neuropeptide and precursor, insulin-like growth factor binding proteins and nerve growth factor binding proteins occurring in the short time points required the use of multiple (3-6) pairs (control versus amphetamine) of arrays for each brain region at each time point. Longer time points showed no alterations due to amphetamine toxicity. It was speculated that the inability to detect changes at the longer time points might have been due partially to a "dilution" factor with the collection of a larger region containing a focal site of injury. In this case, the use of microdissection or laser capture microscopy may enhance the ability to detect subtle changes in focal brain regions.
Genes; Neurotoxicity; Neurotoxic-effects; Neurotoxicology; Microscopy; Neurotoxins