Mutagenicity of 4.4'methylene-bis-2-chloroanaline (MOCA) and 2-phenyl-1.4-benzoquinone (PBQ) in human lympho-blastoid cells.
Reid-TM; DeBord-DG; Cheever-KL; Savage-RE Jr.
Toxicologist 1998 Mar; 42(1-S):80
The genotoxic potential of two occupationally significant chemicals, 4.4'methylene-bis-2-chloroanaline (MOCA) and 2-phenyl-1.4-benzoquinone (PBQ), was explored by monitoring the induction of mutations at the HPRT locus of AHH-l human lymphoblastoid cells. In order to bind to DNA, MOCA requires activation via N-oxidation to N-hydroxy-MOCA (N-OH- MOCA). Exposure of AHH-l cells to N-OH-MOCA induced a 6-fold increase in mutant frequency and resulted in base pair substitutions primarily at A:T base pairs. In contrast, exposure to PBQ did not result in an increased mutant frequency although this compound was significantly more cytotoxic than N- OH-MOCA at equimolar doses. The induction of mutations at A:T sites by N-OH-MOCA is consistent with the type of DNA damage known to be produced by MOCA and provides a specific marker of genotoxic damage for exposed populations.
Genotoxic-effects; DNA-damage; Mutation; Cellular-function; Cellular-structures; Cell-transformation
The Toxicologist. Society of Toxicology 37th Annual Meeting, March 1-5,1998, Seattle, Washington