The role of sensitization routes in the development of type I hypersensitivity to natural rubber latex in B6C3F1 mice.
Woolhiser MR; Meade BJ; Munson AE
Toxicologist 1998 Mar; 42(1-S):268
Latex allergy has become recognized internationally as a serious health hazard. Different allergen-specific IgE profiles have been associated with latex allergic adults as compared to younger patients (e.g. health care workers vs. spina bifida patients). As health care workers are thought to be primarily exposed to latex allergens dermally and by inhalation. and spina bifida children are additionally exposed subcutaneously to latex via numerous surgical procedures, a leading hypothesis is that the route of latex sensitization results in varied allergen-specific IgE repertoires. We are developing murine models representative of these routes of exposure. Preliminary studies were designed to demonstrate the ability of B6C3F1 mice to mount IgE responses to latex proteins. Mice were dosed intranasally with approximately 10 ug of latex protein every fifth day over 52 weeks (9 exposures total). ELISA determinations of total IgE concentrations demonstrated IgE levels which were almost 4-fold higher than those of control mice (3, 500 ng/ml vs. 900 ng/mI). In addition, splenocytes and lymphocytes analyzed by flow cytometry demonstrated an increased expression in B220 (B-cells) and surface IgE. 73% of the B220( + ) lymphocytes from latex treated mice stained positive for surface IgE while only 13.5% did so for vehicle exposed mice. Likewise, 43% of B220( +) splenocytes stained positive for IgE compared to 9% from vehicle mice. In a second study, mice were injected s.c. with 50, 100, or 150 ug of latex proteins on days 1, 8, 15, and 22. Total IgE concentrations reached 5, 000 ng/ml by day 14 for all three latex groups. The 50 ug injections resulted in IgE concentrations on day 28 greater than 10,000 ng/ml while the IgE levels following the 100 ug and 150 ug injections were 7.500 and 5.800 ng/ml respectively. These experiments suggest that the B6C3F I mouse will serve as an acceptable test system to mimic the latex exposure routes which lead to latex hypersensitivity.
Dermatology; Dermatitis; Skin-sensitivity; Skin-protection; Skin-irritants; Skin-infections; Skin-disorders; Skin-diseases; Skin; Health-care-personnel; Gloves; Clothing; Animal-studies; Animals; Laboratory-animals; Laboratory-testing; Hypersensitivity; Medical-personnel
The Toxicologist. Society of Toxicology 37th Annual Meeting, March 1-5,1998, Seattle, Washington