Ovulatory responses of adult mice treated neonatally with estradiol or methoxychlor (technical grade).
Eroschenko-VP; Swartz-WJ; Ford-LC
FASEB J 1994 Apr; 8(5)(II):A959
Methoxychlor (MXC), a widely used organochlorine pesticide is estrogenic. To determine its effects on ovulation, one-day-old mice were exposed daily for 14 days to either sesame oil or 1.0 ug estradiol-17B (E), or 0.1 mg, 0.5 mg, or 1.0 mg MXC suspended in sesame oil. At two and four months of age, the animals were injected with a superovulatory regimen of 10 I.U. of pregnant mare's serum followed by 10 I.U. of human chorionic gonadotropin. The chemical treatments produced both a time- and dose- dependent changes. Ovulatory responses and ovarian weights were significantly reduced by two months of age in mice neonatally exposed to E, 0.5 mg, or 1.0 mg MXC. By four months of age, all treated animals exhibited a significant reduction in number of oocytes ovulated in response to exogenous gonadotropin. The ovaries in the treated mice also exhibited fewer fresh corpora lutea and an increase in the number of atretic follicles. It is concluded that neo natal methoxychlor exposures alter ovarian responses to exogenous gonadotropins, resulting in decreased ovulations.
Reproductive-effects; Reproductive-hazards; Reproductive-system-disorders; Laboratory-animals; Insecticides; Toxic-effects; Pesticides; Pregnancy; Prenatal-exposure; Risk-factors; Exposure-levels; Dose-response
Abstract; Conference/Symposia Proceedings
The FASEB Journal. Experimental Biology '94, Anaheim, California, April 24-28, 1994
Louisiana State University, School of Medicine, Department of Anatomy, New Orleans, Louisiana