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Meeting guidelines for cholinesterase monitoring by clinical laboratories in California.
Wilson BW; Henderson JD; Arrieta DE; O'Malley MA
Toxicologist 2004 Mar; 78(S-1):172-173
California (CA) is the only state with a long standing formal monitoring program for mixers, loaders and applicators of pesticides. When we found commercial clinical kits were not optimal for assaying blood cholinesterases (ChEs), CA regulations were revised to specify use of optimal Ellman ChE assay conditions. Alternate methods were allowed if a correlation (r 2) of 0.9 was achieved in a comparison with the specified method. We were enlisted to work with the clinical laboratories. Only 2 of 7 participating laboratories achieved an acceptable correlation for red blood cell acetylcholinesterase (AChE) and 4 of 5 laboratories for plasma ChE. When the CA Department of Pesticide Regulation (DPR) reiterated the need to meet this requirement we used bovine ghost RBC AChE as a standard to work with several of the clinical laboratories. Only 3 of 10 participating laboratories had acceptable correlations. Some said their instruments lacked the sensitivity to assay the bovine AChE samples. Next, we provided all interested laboratories with human blood and plasma samples to perform a comparison study outlined in the regulation (Section 6728f). Fourteen laboratories participated; 9 met the criterion for whole blood, 14 for plasma and 6 for RBC AChE. Based on such data, DPR notified the CA Agricultural Commissioners on July 8, 2003 that 9 clinical laboratories were approved for ChE testing. We continue to work with laboratories interested in being on the approved list.
Cholinesterase-inhibitors; Monitoring-systems; Clinical-tests; Laboratory-testing; Laboratories; Pesticides; Blood-cells; Red-blood-cells; Agricultural-chemicals; Agriculture; Pesticides-and-agricultural-chemicals; Farmers; Agricultural-workers
The Toxicologist. Society of Toxicology 43nd Annual Meeting and ToxExpo, March 21-25, 2004, Baltimore, Maryland
University of California - Davis
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Content source: National Institute for Occupational Safety and Health Education and Information Division