Dermal exposure to JP-8 jet fuel during pregnancy alters immunological function in F1 mice.
Keil-DE; Butterworth-L; Azadi-S; Peden-Adams-M
Toxicologist 2004 Mar; 78(S-1):98
Approximately 5 billion gallons of JP-8 jet fuel (JP-8) are used annually by the United States Department of Defense (DOD) making this the single largest chemical exposure to military personnel. In addition, the aviation industry extensively uses commercial jet fuel which is essentially JP-8 without certain additives for anti-icing, corrosion, and static dissipation. Civilian exposure can occur as well through environmentally contaminated sites where jet fuel has been reportedly spilled or leaked from supply lines or storage tanks. Many reports indicate that JP-8 alters immunological function in adult rodents. However, little is known regarding its impact on the developing immune system. Therefore, C57BL/6N pregnant dams (mated with C3H/HeJ males) were dermally exposed with 10, 25, 50, or 75 uL neat applications of JP-8 daily during gestation days 6-15. F1 offspring were evaluated for immunological alterations at 3 and 8 weeks of age. There were no treatment effects on body, liver, kidney, or thymus mass. Although slight, an increase in spleen mass and cellularity was observed at both 3 and 8 weeks. T-cell proliferation following anti-CD3 stimulation was not affected at either age. However, IgM plaque forming cell (PFC) responses were significantly suppressed in 3 week old pups exposed to 50 or 75 uL JP-8 and in all treatment groups at 8 weeks of age. Our data indicate that prenatal dermal exposure to JP-8 can affect the developing murine fetus resulting in impaired humoral immune responses detectable at adult-hood.
Jet-engine-fuels; Pregnancy; Exposure-levels; Immunologic-disorders; Laboratory-animals; Animals; Animal-studies; Military-personnel; Environmental-contamination; Environmental-exposure; Immune-system-disorders; Teratogenesis; Teratogens
The Toxicologist. Society of Toxicology 43nd Annual Meeting and ToxExpo, March 21-25, 2004, Baltimore, Maryland