Comparative cytotoxic effects of crocidolite and its non-asbestiform polymorph on rat alveolar macrophages.
Castranova-V; Pailes-WH; Judy-D; Schwegler-Berry-D; Jones-W
Ann Occup Hyg, Inhaled Particles VII 1994 Jan; 38(Suppl 1):665-673
The objective of this study was to compare the cytotoxicity of an asbestiform mineral, crocidolite, to that of its non-asbestiform analogue, riebeckite. Crocidolite had a median fibre length of 11.5 Jim and a surface area of 17 m2 g -1 while the count median diameter of riebeckite was 3 um with a surface area of2.7 m2 g-i. Crocidolite samples contained 20--40 times more particles per mass than riebeckite samples. Cytotoxicity was determined by treating rat alveolar macrophages in culture with various concentrations of particles for 1-3 days and monitoring membrane integrity ,leakage of cytoplasmic and lysosomal enzymes and zymosan-stimulated respiratory burst activity. In general, crocidolite exhibited cytotoxicity at doses as low as 100 ug ml-1. Riebeckite was cytotoxic in most assays at 300 ug ml- 2. Therefore, on a mass concentration basis, crocidolite was more toxic than riebeckite. On an equivalent surface area basis, riebeckite was slightly more toxic than crociodolite. On an equivalent particle count basis, riebeckite was far more toxic.
Cytotoxic-effects; Cytotoxicity; Cytotoxins; Minerals; Samplers; Animals; Animal-studies; Fibrous-bodies
V. Castranova, Division of Respiratory Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
Annals of Occupational Hygiene, Inhaled Particles VII