Detection of n-formyl-methionyl-leucyl-phenylalanine (FMLP) in cotton dust: biological activities of FMLP associated with pulmonary responses to cotton dust exposure.
Fedan-JS; Ma-JKH; Frazer-DG; Mo-CG; Castranova-V
Ann Occup Hyg, Inhaled Particles VII 1994 Jan; 38(Suppl 1):879-885
Inhalation of cotton dust (20 mg m - 3 for 6 h) by guinea pigs is marked by infiltration of granulocytes into the airways (11. 7-fold) and airway closure (I.3-fold). In addition, aqueous extracts of cotton dust cause constriction of tracheal smooth muscle in vitro. Analysis of water or acetone extracts of cotton dust by HPLC reveals substantial quantities (mg g-l) of both FMLP and oxidized FMLP. As with cotton dust extracts, FMLP is an agonist of guinea pig tracheal smooth muscle (ECso=91 nM FMLP). Both reduced and oxidized FMLP stimulate chemotaxis and respiratory burst activity in granulocytes. Furthermore, activated pulmonary granulocytes isolated from cotton dust-exposed guinea pigs induce contraction of trachealis muscle in vitro. Thus, FMLP may be involved in the pulmonary response to cotton dust exposure directly by causing airway constriction and indirectly by inducing inflammation characterized by granulocyte infiltration and activation.
Cotton-dust; Dust-exposure; Dust-analysis; Dust-inhalation; Animals; Animal-studies; In-vitro-study; Laboratory-animals; Pulmonary-system
J. S. Fedan, Division of Respiratory Disease Studies, National Institute for Occuaptional Safety and Health, Biochemistry Section, 994 Chestnut Ridge Road, Morgantown, WV, 26505, USA
Annals of Occupational Hygiene, Inhaled Particles VII