Respirable quartz loss of an absorbed pulmonary surfactant in vitro and expression of cytotoxicity or genotoxicity.
Liu-X; Keane-MJ; Ong-T; Antonini-JM; Wallace-WE
Ann Occup Hyg, Inhaled Particles VIII 1997 Jan; 41(Suppl 1):415-419
Inhaled particles will contact a surfactant-rich surface hypophase upon deposition in a pulmonary alveolus. Interactions with surfactant may affect the expression of particulate toxicity and may be a factor in distinguishing the pathogenic potentials of different dusts. Research has found that dipalmitoyl phosphatidylcholine (DPPC). a primary component of that surfactant. in aqueous dispersion will absorb onto the surface of respirable sized quartz particles and promptly suppress membranolytic activity (Wallace et al.. 1985. 1988). Cell-free systems have been used to investigate phospholipase enzymatic digestion of quartz-adsorbed DPPC, and the resultant surfactant removal and restoration of dust surface cytotoxicity (Wallace et al., 1992; Keane and Wallace. 1995). Research reported here examines the expression of in vitro toxicity with time after challenge of pulmonary macro- phage by DPPC-coated quartz dust, of the expression of micronucleus activity by a similarly challenged V79 cell line. and of the use of fluorescence-label substituted DPPC with dioleoyl-phosphatidyl choline (DOPC) adsorbed on quartz particles to track the removal of surfactant from quartz particles within cells.
Inhalants; Airborne-particles; Surfactants; Surface-properties; Pulmonary-disorders; Pulmonary-function; Particulate-dust; Particulates; Dust-inhalation; Dust-exposure; Dusts; Dust-particles; Quartz-dust; Aerosols
National Institute for Occupational Safety and Health (NIOSH), 1196 Willowdale Road, Morgantown, WV 26505, USA
Annals of Occupational Hygiene, Inhaled Particles VIII