Induction of unscheduled DNA synthesis in V79 cells by diesel emission particles dispersed in simulated pulmonary surfactant.
Gu-ZW; Zhong-BZ; Keane-MJ; Whong-WZ' Wallace-WE; Ong-T
Ann Occup Hyg, Inhaled Particles VII 1994 Jan; 38(Suppl 1):345-349
Abstract-Diesel emission particles (DEP) from an engine operating on the Federal Test Procedure (DEP) were dispersed in an aqueous mixture of dipalmitoyllecithin (DPL). a major component of pulmonary surfactant and saline and were tested for genotoxicity using the unscheduled DNA synthesis (UDS) assay with autoradiography techniques In cultured Chinese hamster lung cells. A DEP sample from the same source was dispersed in dimethyl sulphoxide (DMSO) and subjected to the same assay Both dispersions in (DPL and DMSO) were found to induce UDS in a dose-related manner. After separation of the sample into supernatant and sediment fractions. the activity of the DEP sample was found to reside In the supernatant fraction for the DMSO-dispersed sample; and in the sedimentary fraction for the surfactant-dispersed sample. These findings further indicate that genotoxic activity associated with DEP inhaled into the lung may become bioavailable through the dispersion properties of pulmonary surfactant components.
Diesel-emissions; Diesel-exhausts; Diesel-engines; Pulmonary-system; Pulmonary-function; Genotoxic-effects
Division of Respiratory Studies, National Insitute for Occupational Safety and Health. Morgantown. WV 26505-2888. USA
Annals of Occupational Hygiene, Inhaled Particles VII