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Procedure for the quantification of the biomarker (2-methoxyethoxy)acetic acid in human urine samples.
B'Hymer C; Cheever KL; Butler MA; Brown KK
J Chromatogr B 2003 Sep; 795(1):145-150
An accurate and precise procedure was developed for the detection and quantification of (2-methoxyethoxy)acetic acid (MEAA), a metabolite and biomarker for human exposure to 2-(2-methoxyethoxy)ethanol. The compound 2-(2-methoxyethoxy)ethanol has a wide array of industrial applications including its use as an additive in military jet fuel. Exposure to 2-(2-methoxyethoxy)ethanol is a health concern owing to its toxicity which includes developmental and teratogenic properties. Sample preparation consisted of liquid-liquid extraction (LLE) and esterification of MEAA to produce the ethyl ester. Measurement was by a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a HP-1 capillary column. Recovery studies of spiked blank urine demonstrated good accuracy and precision; recovery varied between 95 and 103% with relative standard deviations of 8.6% and less. The limit of detection (LOD) for this procedure was found to range from 0.02 to 0.08 ug/ml equivalent levels of MEAA in urine. These data and other aspects of the validation of this procedure will be discussed.
Acetic acids; Ethanols; Metabolites; Gas chromatography; Jet engine fuels; Developmental disorders; Teratogenesis; Esters; Gas detectors; Urinalysis; Author Keywords: (2-Methoxyethoxy) acetic acid
National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
Issue of Publication
Research Tools and Approaches: Exposure Assessment Methods
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Page last reviewed: April 9, 2021
Content source: National Institute for Occupational Safety and Health Education and Information Division