Gene expression profile in rat alveolar macrophages stimulated with silica in vitro or in vivo.
FASEB J 2003 Apr; 17(7):C294
Expression of several cytokines at the message level was studied in rat alveolar macrophages stimulated with silica in vitro or in vivo, using real-time RT/PCR. Alveolar macrophages were isolated from male Sprague-Dawley rats by bronchoalveolar lavage 4 h following intratracheal instillation of either silica (2 mg silica/100 g body weight) (or an equivalent volume of PBS (vehicle). For in vitro experiments, alveolar macrophages were stimulated with silica (200 ug/ml) for 4 h. Total RNA was isolated using RNAqueousTM-4PCR kit (Ambion) and reverse transcribed using ThermoScriptTM (Invitrogen). Real-time PCR was performed using SYBR green kits. The table below shows the results. The data indicate that cytokine production in alveolar macrophages simulated with silica is regulated by different factors. The differences seen in in vitro and in vivo experiments indicate that the production of some of the cytokines depends upon the interaction of products secreted by different cell types.
Silicates; In-vitro-study; In-vivo-study; Cell-function; Ribonucleic-acids; Animal-studies; Laboratory-animals; Genetic-factors
Abstract; Conference/Symposia Proceedings
The FASEB Journal, Immunology 2003, Denver, Colorado May 6-10, 2003