Induction of apoptosis from instillation of agricultural dust.
Mercer-RR; Wang-L; Antonini-JM; Scalilloni-JF; Vallyathan-V; Castranova-V
FASEB J 2002 Mar; 16(5):A962
In this study, we test the hypothesis that induction of alveolar macrophages (AM) apoptosis plays a role in lung injury from particulates. We examined AM apoptosis in F344 rats following intratracheal instillation of 1ml of saline containing 4mg of particles. Particles used include crystalline silica, titanium dioxide and particulates from air sampling of citrus and grape fields. After 4 weeks, rats were sacrificed and lung sections analyzed for the number of apoptotic cells per lung using TdT labeling. Apoptotic macrophages, expressed in millions of cells per lung (mean+SE,N=4) were 43.4+4 for crystalline silica, 10.50+0.2 for grape, 8.1+0.5 for citrus and 3.1+0.4 for titanium dioxide. Saline controls were 1.6+0.4. The degree of apoptosis correlated well with inflammation and damage suggesting a role for apoptosis in lung injury resulting from particulates.
Lung-function; Lung-disorders; Particulates; Alveolar-cells; Animal-studies; Silicates; Air-samples; Laboratory-animals; Respiratory-system-disorders; Pulmonary-system-disorders; Agriculture; Dust-exposure; Dust-particles; Dusts; Agricultural-industry; Particulate-dust; Silica-dusts; Air-sampling; Animals; Lung-irritants
NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
Abstract; Conference/Symposia Proceedings
The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002