Alternation of innate and cell-mediated immunity to Listeria monocytogenes by short-term exposure to diesel exhaust particles.
Yin-XJ; Schafer-R; Antonini-JM; Marger-MW; Dong-CZ; Roberts-JR; De La Rosa-P; Ma-JYC; Ma-JKH
FASEB J 2002 Mar; 16(5):A962
The effects of diesel exhaust particles (DEP) on functions of pulmonary host defense were Studied using a rat Listeria infection model. Short-term DEP inhalation (SO and 100 mg'm3, 4 h) by rats resulted in a slowed lung bacterial clearance, suppressed alveolar macrophage (AM) phagocytosis and reduced AM production of tumor necrosis factor (TNF)-alpha. interleukin (IL)-1 and IL-12 in response to Listeria. The combined DEP/Listeria exposure was also characterized by increased CD4+ and CD8+ cell counts, and percentage of CD8+ cells in lung draining lymph nodes. The lymphocytes from DEP-treated rats showed increased IL-2 production when challenged with concanavalin A (ConA). Cells from the combined exposed rats produced increased interferon (IFN)-gamma at day 7, but decreased IFN-gamma at day 3, when challenged with either ConA or heat-killed Listeria (HKLM). Listeria significantly induced lymphocyte secretion of IL-6 in response to HKLM. which could be increased by DEP preexposure. These results indicate that short-term DEP exposure aggravates Listeria infection by suppressing AM phagocytosis and the production of TNF-alpha, IL-I and IL-12, and by eliciting an adverse effect on T -cell-mediated immunity.
Diesel-exhausts; Pulmonary-function; Pulmonary-system; Animal-studies; Lung-function; Lung; Phagocytic-activity; Phagocytes; Laboratory-animals; Diesel-emissions; Particulates
Abstract; Conference/Symposia Proceedings
Work Environment and Workforce: Mixed Exposures
The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002