Influence of hyperthyroidism on rat lung cytokine production and nuclear factor (NF)-KappaB activation following ozone exposure.
Huffman-LJ; Prugh-D; Brumbaugh-K; Ding-M
FASEB J 2002 Mar; 16(5):A961-A962
Results from previous studies indicate that hyperthyroidism increases the risk of ozone-induced lung toxicity. In the present study, we evaluated cytokine levels in bronchoalveolar lavage fluid from control and hyperthyroid male rats 18 hours after exposure to air or ozone (2 ppm for 3 hours). MIP-2 and MCP-I levels were increased in both control and hyperthyroid rats following ozone. However, the increase in hyperthyroid rats were much greater, MIP-2 (I.S fold) and MCP-I (II fold), when compared to levels in controls. These changes appeared to be relatively specific; bronchoalveolar lavage fluid levels of IL-6, IL-4, and IL-IO were generally low or non-detectable across all groups at this time. We also found that NF-kappa B binding activity was increased at both 4 and 18 hours following ozone exposure in bronchoalveolar lavage cell extracts from hyperthyroid rats relative to controls. Collectively, these results suggest that mechanisms contributing to the enhanced pulmonary inflammatory response to ozone in a hyperthyroid state include an increase in NF-Kappa B activation and an up-regulation of chemokine production.
Lung-irritants; Lung-disorders; Lung-function; Lung-tissue; Animal-studies; Pulmonary-system; Pulmonary-function; Pulmonary-system-disorders; Laboratory-animals; Respiratory-system-disorders; Endocrine-system-disorders; Exposure-levels; Environmental-exposure; Toxic-effects; Lung-disease; Air-monitoring
Abstract; Conference/Symposia Proceedings
The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002