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The effects of single and serial zinc oxide exposures on airway inflamation in healthy humans.
Reinties-K; Witten-A; Arjomandi-M; Balmes-J; Blanc-P; Solomon-C
Am J Respir Crit Care Med 2002 Apr; 165(8)(Suppl):A46
A single exposure to zinc oxide (ZnO) results in an increase in inflammatory cells and cytokines. Serial exposures to ZnO can decrease this inflammatory response. This experiment was designed to test the hypothesis that exposure to ZnO on two consecutive occasions would decrease both the cellular and cytokine responses compared 10 one exposure. Utilizing a repeated measure, randomized, single-blind design, 6 healthy subjects (4 females; age range 26-53 yr; methacholine PC,. > 10 mg/ml) were exposed for 30 min, at rest, to: I) filtered-air (FA); 2) ZnO on one occasion (ZnO-I: conc: 10 mg/ m'); 3) ZnO on two consecutive occasions (ZnO-2: conc: 10 mg/m'). Sputum induction (3% saline, t=20 min) was performed 65 h pre- and 6 h post-exposure, Conditions were compared using the post minus pre delta value. In ZnO-I, compared to FA, there was a significant (P<O.05) increase in the total leukocyte concentration (median; 25-75% range: 16.6; 7.5-23.0 vs -0.3; 0.3--0.8 x 10' cells/ml), and TNFa(I.4; 0.6-6.5 vs 0.6; -0.1-1.8 pg/ml). In ZnO-2, compared to FA, there was a significant increase in TNFa (2.8; 1.0- 7.0 vs 0.6; -0.1-1.8 pg/ml). For all three conditions there were no other differences in total leukocyte concentration, or macrophage, Europhile, lymphocyte, or eosin Phil differential percent, or in total protein. TNFa, or IL-8. These results indicate that serial exposure to ZnO does not result in the increase in leukocytes observed for a single exposure, but that serial exposure does not change the TNFa response.
Zinc-compounds; Filters; Filtration; Sputum-analysis; Sputum-cytology; Leukocytes; Proteins; Exposure-limits; Exposure-levels
Issue of Publication
Research Tools and Approaches; Exposure Assessment Methods
American Journal of Respiratory and Critical Care Medicine, 2002 International Conference, The American Thoracic Society, Atlanta Georgia, May 17-22, 2002
Center for Occupational and Enviromental Health, University of California, Berkley California
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division