Biomarker characterization of asphalt fume exposure.
Wang-JJ; Lewis-DM; Law-B; Frazer-DG; Stone-S; Goldsmith-T; Mosley-A; Simpson-J; Siegel-PD
Toxicologist 2002 Mar; 66(1-S):284
Each year more than 60 million tones of asphalt are produced and used worldwide. It has been estimated that approximately two million workers were employed and potentially exposed to asphalt. Health concern from exposure to asphalt is related to its content of hazardous chemicals, such as polycyclic aromatic hydrocarbons (PAHs). The development of a biomarker to provide an assessment of the integrated external exposure following uptake by living system through inhalation is valuable for this kind of complex mixture exposure. Many PAHs are metabolized to hydroxyl-PAHs and excreted in urine; therefore, urinary hydroxyl-PAH was used as a biomarker for asphalt fume exposure in this study. Female Sprague-Dawley rats and B6C3F1 mice were exposed to asphalt fume in a whole body inhalation chamber for 5 or 10 days (3.5 hrs/day, 71-97 mg/m 3 ). Clean air chamber animals were used as controls. The test asphalt was the type used by paving industry throughout the Midwestern United States. The fume was generated under simulated road paving conditions. Urine sample preparation for the analysis of hydroxyl-PAHs metabolites included enzymatic digestion, solid phase extraction, and isotope dilution GC/MS and microflow LC-Q-TOF MS detections. Four priorities PAH metabolites, including, 1-hydroxypyrene, 9-phenanthrenol, 1-chrysenol, and 2-naphthalenol were identified and determined in urine. Isotope-dilution has proved to be useful for quantification of those metabolites. Preliminary results indicated that urinary PAH metabolite could be a sensitive biomarker following asphalt fume exposure. The method developed in this study has potential application to monitor of PAHs from occupational mixture exposure.
Asphalt-fumes; Humans; Workers; Exposure-levels; Inhalants; Urine-chemistry; Animal-studies; Laboratory-animals; Urinalysis; Urine-chemistry; Analytical-chemistry
Work Environment and Workforce: Mixed Exposures
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee