Restraint stress modulation of ear swelling in ACD is independent of sensitizing dose of chemical.
Flint MS; Salmen RR; Brumbaugh K; Tinkle SS
Toxicologist 2002 Mar; 66(1-S):241
Stress plays an important role in numerous chronic health problems including modulation of immunologic diseases, however, the interaction between the stress response and the cutaneous immune response are incompletely understood. We have previously shown that acute stress suppresses the immune response in allergic contact dermatitis (ACD), and additional studies have shown a significant interaction between the concentration of chemical required for sensitization and for challenge in the development of ACD. We hypothesized that 2 hour restraint stress would change the sensitizing concentration of chemical required to elicit a full ear swelling response to chemical challenge. Male BALB/c mice were exposed on the back with increasing doses (0.05%-0.5%) of 2, 4 dinitrofluorobenzene (DNFB) on days 1 & 2 and challenged onto the ear with 0.1% DNFB on day 6. Mice were restrained for 2 hours prior to chemical application on day 1. We assessed ear swelling 24, 48 and 72 h post-challenge. Quantitative analysis of the data indicated that the ear swelling response to chemical challenge is dependent upon the sensitization concentration of DNFB and is dose-responsive. The curve shows a quadratic trend with 0.1% as a possible maximum dose for sensitization. Application of restraint stress did not alter the shape of this curve but significantly suppressed ear swelling at all concentrations of DNFB. These data suggest that restraint modulation of ACD is independent of the sensitizing dose of chemical.
Stress; Immunologic-disorders; Immune-reaction; Allergic-dermatitis; Sensitization; Animal-studies; Laboratory-animals
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee