A novel technique to study percutaneous absorption by using a silastic membrane coated fiber.
Xia-XR; Baynes-RE; Monteiro-Riviere-NA; Leidy-RB; Riviere-JE
Toxicologist 2002 Mar; 66(1-S):164
Silastic membrane coated on a section of fused silica fiber has been used successfully as an analytical tool to extract analytes from aqueous solution. In this study, the silastic membrane coated fiber was used as a permeation membrane to study the percutaneous absorption of model pesticides. It represents a half compartment of the conventional Franz diffusion cell, which allows for more detailed permeation kinetics to be investigated. The membrane coated fiber was immersed in the magnetic stirred donor phase to adsorb the permeants from the solution, the fiber was then transferred directly into the injection port of a Gas Chromatograph/Mass Spectrometer for quantitative analysis. This technique can assess the permeation amounts at various permeation times, stirring speeds and donor compositions. A theoretical model was proposed to describe the permeation processes of the silastic membrane coated fiber in terms of diffusion parameters. This model allows for the boundary layer between the donor phase and the silastic membrane to be considered, it's thickness is assumed to be constant under steady-state diffusion. The permeation kinetics of 30 different compounds having a wide range of partition coefficients were examined utilizing a silastic membrane coated fiber. The experimental permeation flux and time profiles were well described by the proposed mathematical model. The thickness of the boundary layer, the diffusion coefficients in the donor phase and in the silastic membrane, and the partition coefficients can be measured simultaneously with this novel technique. This approach would foster development of high-throughput determination of skin diffusion parameters.
Skin-absorption; Diffusion-analysis; Fiber-deposition; Magnetic-fields; Gas-chromatography; Mass-spectrometry
Disease and Injury: Allergic and Irritant Dermatitis; Work Environment and Workforce: Mixed Exposures
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
North Carolina State University, Raleigh, NC and Pesticide Residue Research Laboratory, Department of Toxicology, North Carolina State University, Raleigh, NC