Hisopathologic assessment of acute dermal exposure to m-xylene, d-limonene and sodium lauryl sulfate in rats.
Brinkley-WW; Rogers-JV; Kabbur-MB; Garrett-CM; Geiss-KT; Gunasekar-JN; McDougal-JN
Toxicologist 2002 Mar; 66(1-S):162
Skin exposure to organic chemicals and solvents may cause skin irritation and generate proinflammatory mediators that initiate an irritant response. Histopathology can serve as a fixed reference point in a changing microenvironment for explanation of concurrent cellular responses. Changes in the levels of selected proteins in the irritant cascade were quantified after an exposure and compared to visible tissue changes. Here we describe the dermal pathology of male F-344 rats exposed to meta-xylene, d-limonene and sodium lauryl sulfate for one hour using Hill Top Chambers . Skin samples were collected at zero, one, two, four and six hours after the end of the exposure. Light microscopic evaluation was performed on formalin-fixed and paraffin embedded skin sections. Hematoxylin and eosin stained sections were assessed and scored. A single topical exposure to meta-xylene, d-limonene, and sodium lauryl sulfate resulted in granulocyte infiltration in rats as early as 2 hours. Segmental detachment of the epidermis from the dermal interface occurred in prolonged exposures of meta-xylene and d-limonene compared to the control groups. These findings were interpreted to be the consequences of potential up-regulation of inflammatory mediators that result in skin damage.
Dermatitis; Irritants; Histopathology; Animal-studies; Allergic-dermatitis; Skin-diseases; Skin-disorders; Skin-irritants; Dermatosis; Laboratory-animals
108-38-3; 5989-27-5; 151-21-3
Disease and Injury: Allergic and Irritant Dermatitis
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
Wright State University, Dayton, Ohio